中国临床心理学杂志

不同时段睡眠剥夺对大鼠脑内c-fos表达的影响

作者：宋国萍皇甫恩苗丹民冯学文

关键词：大鼠 c-fos蛋白大脑睡眠剥夺

分类号：B845.1

出版年,卷(期):页码：2002,10(2):103-105

摘要：

目的:探索睡眠剥夺的脑机制。方法:该研究采用小平台水环境法建立大鼠睡眠剥夺模型,用fos蛋白免疫组化的方法测量脑中fos蛋白的表达,分组为白天睡眠剥夺12h组、夜晚睡眠剥夺12小时组、大平台对照组和正常单独饲养组,每组4只。结果:睡眠剥夺使fos蛋白在皮层的广泛区域表达,脑干中同异相睡眠有关的区域有较高表达,同夜晚睡眠剥夺12小时相比,白天睡眠剥夺12小时在视交叉上核同生物节律有关的区域表达。结论:睡眠节律改变可影响脑内fos蛋白表达

Objective:To investigate the brain mechanisms of sleep deprivation.Methods:Sprague-Dawley rats were sleep deprived by housing them on the small platforms over water.Controls were housed in the normal cage(CC) or on the large platforms over water(TC).The effects of SD on the expression of c-fos protein by immunocytochemistry were investigated.The duration of SD was about 12 hours.They were divided into two groups:early SD group (from 7:00 am) and the late SD group (from 19:00 pm).Results:SD resulted in different expression of c-fos protein at different fields of the brain.The main fields of brain stem were all connected with paradoxical sleep.Compared with the late SD,the early SD group showed higher fos protein expression on suprachiasmatic.Conclusion:The disruption of regular circadian rhythm may affect c-fos protein expression.

基金项目：

作者简介：

参考文献：

[1] Novak CM,Nunez AA.Daily rhythms in fos activity in the rat ventrola teral preoptic area and midline thalamic nuclei,Am J Physiol,1998,275:1620-1626
[2] 宋国萍,皇甫恩,苗丹民,等.连续不同时间睡眠剥夺后大鼠脑干中c-fos蛋白的表达.中国行为医学科学,2001:10:407-409
[3] Bradley DY,Zhou J,Smagin GN,et al.Sleep deprivation by the "Flower Pot" technique and spatial reference memory.Physiol Behav,1997,2:249-256
[4] O'Hara BF,Young KA,Watson FL,et al.Immediate early gene expression in brain during sleep deprivation:Preleminary observations.Sleep,1993,1:1-7
[5] 宋国萍,苗丹民,皇甫恩,等.睡眠剥夺对大鼠学习和行为的影响.第四军医大学学报,2000,6:663-666
[6] Tobler I,Borbely AA.The effect of 3-h and 6-h sleep deprivation on sleep and EEG spectra of rat.Behav Brain Res,1990,6:73-78
[7] Watanbe Y,Stone E,McEwen BS.Induction and habituation of c-fos and Zif/286 by acute and repeated stressor.Neuro Report,1994,5:1321-1224
[8] Tononi G,Pompeiano M,Cirelli C.The locus coeruleus and immediate-eraly genes in spontaneous and forced wakefulness.Brain Res Bull,1994,5-6:589-596
[9] Lydie R,Baghdoyan HA,Hibbard EV,et al.Regional brain glucose metabolism is al tered during rapid eye movement sleep in the cat:a priliminary study.J Comp Neur ol,1991,2:517-529
[10] Hugo MN Jacqueline V,Fabio C et al.Brain distribution of c-fos expression as a result of prolonged rapid eye movement (REM)sleep period duration.Brain Res,1995,1:15-22
[11] Pompeiano M,Cirellie C Tononi G.Effects of sleep-deprivation on fos-like immunoreactivity in the rat brain.Arch Ital.Biol,1992,3:325-335
[12] Daan S,Beersma DGM,Borbely AA.The timing of human sleep:recovery process gated by a circadian pacemaker.Am J Physiol,1984,2:R161-178
[13] Merchant NH,Vazquez J,Garcia F,et al.Brain distribution of c-fos expression as a result of prolonged rapid eye movement(REM) sleep period duration.Brain Re s,1995,68:15-22
[14] Margarita M,Ignacio M.Improvement of shuttle-box avoidance following post-training treatment in paradoxical sleep deprivation platforms in rats.Physi ol＆Behavior,1988,43:93-98.

服务与反馈：

【文章下载】【加入收藏】