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5-羟色胺转运体基因启动子区CpG岛甲基化状态与精神分裂症Ⅰ型和Ⅱ型的关联
作者:张海生1 2  王伟1  余道军3  张顺泉2  谢健2 
单位:1. 浙江大学医学部临床心理室  浙江杭州310058 
2.
 杭州市第一人民医院临床心理科  浙江杭州310006 
3.
 杭州市第一人民医院检验科 
关键词:精神分裂症 甲基化 5-羟色胺转运体(5-HTT) 
分类号:R749.3
出版年,卷(期):页码:2011,19(3):289-292
摘要:

目的:探讨5-羟色胺转运体(5-HTT)基因启动子区CpG岛甲基化状态与精神分裂症Ⅰ型和Ⅱ型的关联。方法:运用特异性甲基检测PCR和直接测序法对62例精神分裂症Ⅰ型患者、38例Ⅱ型患者和50例健康被试5-HTT基因启动子区CpG岛甲基化状态进行检测。结果: 三组5-HTT基因启动子区CpG岛甲基化阳性率无显著性差异;精神分裂症Ⅰ型患者5-HTT基因启动子区CpG岛内位点甲基化率显著高于精神分裂症Ⅱ型患者和健康被试组。结论:5-HTT基因启动子区CpG岛内位点高甲基化可能是精神分裂症Ⅰ型的发病机制之一。

Objective:To explore the relationship between methylation status of CpG islands in the promoter region ofserotonin transporter gene(5-HTT) and type I or II schizophrenia.Methods:Methylation Specific PCR and DNA sequencing were used to detect the methylation status of CpG islands in 62 type I schizophrenia patients,38 type IIschizophrenia patients and 50 health subjects.Results:The methylation rates of CpG islands within the promoter region of5-HTT of the three groups are 52%(32/62),47%(18/38) and 50%(25/50),respectively.The methylation rates of loci inCpG islands within the promoter region of 5-HTT in type I schizophrenia group is significantly higher than those in type IIschizophrenia group and health subjects.Conclusion:The high methylation rate of loci in CpG islands within the promoter region of 5-HTT should be a possible factor to influence the nosogenesis of type I schizophrenia.

基金项目:
杭州市科技计划项目(20090833B06)
作者简介:
参考文献:

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