中国临床心理学杂志

慢性应激对小鼠学习记忆及免疫细胞的影响

作者：马颖¹ 卢莉² 薛朝霞² 袁洁²

单位：1. 山西医科大学第一医院神经外科山西太原 030001
2. 山西医科大学医学心理学教研室山西太原 030001

关键词：慢性应激学习记忆免疫细胞

分类号：R395.1

出版年,卷(期):页码：2009,17(4):394-395

摘要：

目的：观察慢性应激对小鼠学习记忆的影响以及脾组织中T细胞亚群CD4、CD8水平的变化。方法：采用束缚制动的方法建立慢性应激模型，应激结束后采用Morris水迷宫实验观察应激状态下小鼠的学习记忆能力；处死，采用流式细胞仪检测小鼠脾组织中CD4+T细胞、CD8+T细胞的数量以及CD4/CD8比值。结果：应激组小鼠在Morris水迷宫中逃避潜伏期较对照组显著延长(P＜0.01)，第一象限的游泳时间比例显著小于对照组(P＜0.01)，小鼠脾淋巴细胞悬液中CD4+T细胞明显低于对照组(P＜0.05)，而CD8+T细胞高于对照组(P＜0.05)及CD4/CD8比值低于对照组(P＜0.05)。结论：慢性应激可以导致机体学习记忆能力减退，抑制细胞免疫功能，容易导致机体产生疾病。

Objective: To observe the effect of chronic psychological stress on change of function of learning and memory, and CD4, and CD8 in mice. Methods: 50 BALB/c mice were randomly divided into control group and immobilization stress group (n=25 in each group). The mice mode of stress was made by restraint for 6 hours per day. Suspend tail test was performed on change of behaviors in mice. RIA was employed to measure levels of neuropeptide Y in the brain. Results: Compared with the control group, the mean escape latency of the mice in the chronic restraint groups was longer than that of the control mice (P<0.01 ), the average percentages of the swimming time in the platform quadrant in the restraint group were less than those in the control group (P<0.01 ). Chronic stress significantly decreased levels of CD4 (P<0.05), and CD4/CD8 (P<0.05), while CD8 increased. Conclusion: Chronic psychological stress can lead to learning and memory dimish, and inhibit the cellular immune function, thus the body easily lead to a disease.

基金项目：

山西省自然科学基金资助项目(051089)

作者简介：

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